Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Apaf1
Entrez Gene ID: 11783
Gene Name: apoptotic peptidase activating factor 1
Gene Aliases: 6230400I06Rik, Apaf-1, Apaf1l, fog, mKIAA0413
Chromosome Location: Chr.10: 90989311 - 91082743 on Build GRCm38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
GenBank mRNA	AK053432.1	-	1 - 1	3351	74

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	AU258882.1	-	1	149		Chr. 10 - 91038092 - 91038435 [-] on Build GRCm38	74
GenBankmRNA	BB539201.2	-	1	286		Chr. 10 - 91037936 - 91038572 [-] on Build GRCm38	74

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	ankyrin repeat and sterile alpha motif domain containing 1B	Anks1b	AIDA-1b, C030032C09Rik, E530015N03Rik, ENSMUSG00000078431, Gm10937, Gm1555	-64.6kb	Anti-sense	Chr. - 89873509 - 90973300 on Build GRCm38
Downstream	IKBKB interacting protein	Ikbip	1200009F10Rik, 1700023M03Rik, D18354, Ikip	41.4kb	Anti-sense	Chr. - 91083039 - 91102613 on Build GRCm38

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