Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Enah
Entrez Gene ID: 13800
Gene Name: enabled homolog (Drosophila)
Gene Aliases: Mena, NDPP-1, Ndpp1, WBP8
Chromosome Location: Chr.1: 181896384 - 182019980 on Build GRCm38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001083120.2	NP_001076589.1	14 - 14	8631	86
	NM_001083121.2	NP_001076590.1	14 - 14	7899	86
	NM_008680.3	NP_032706.2	15 - 15	8643	86
	NM_010135.3	NP_034265.2	15 - 15	8688	86
GenBank mRNA	AK081526.1	-	1 - 1	1853	86

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	CB519783.1	-	1	498		Chr. 1 - 181899776 - 181900530 [-] on Build GRCm38	86
GenBankmRNA	AW121512.1	-	1			Chr. 1 - 181899793 - 181900360 [+] on Build GRCm38	86
GenBankmRNA	CK639844.1	-	1	196		Chr. 1 - 181899451 - 181900228 [-] on Build GRCm38	86
GenBankmRNA	CB244257.1	-	1	491		Chr. 1 - 181899821 - 181900523 [-] on Build GRCm38	86

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	lamin B receptor	Lbr	AI505894, ic	-57.5kb	Sense	Chr. - 181815315 - 181842401 on Build GRCm38
Downstream	signal recognition particle 9	Srp9	9kDa	222.9kb	Anti-sense	Chr. - 182124737 - 182132415 on Build GRCm38

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