The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_024171.2 | NP_077133.1 | 1 - 1 | 47 | 98 |
| GenBank mRNA | AK004505.1 | - | 1 - 1 | 48 | 98 |
| AK008739.1 | - | 1 - 1 | 51 | 98 | |