The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_017372.3 | NP_059068.1 | 1 - 1 | 153 | 102 |
| GenBank mRNA | AK148516.1 | - | 1 - 1 | 154 | 102 |
| AK150998.1 | - | 1 - 1 | 156 | 102 | |
| AK153244.1 | - | 1 - 1 | 156 | 102 | |
| AK153475.1 | - | 1 - 1 | 154 | 102 | |
| AK159276.1 | - | 1 - 1 | 203 | 102 | |
| AK159640.1 | - | 1 - 1 | 150 | 102 | |
| BC002069.1 | - | 1 - 1 | 145 | 102 | |
| BC054463.1 | - | 1 - 1 | 145 | 102 | |