Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: LIG4
Entrez Gene ID: 101116239
Gene Name: DNA ligase 4
Gene Aliases: -
Chromosome Location: Chr.10: 93681348 - 93688211 on Build Oar_rambouillet_v1.0

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001318075.1	NP_001305004.1	1 - 1	2621	111
	XM_012184941.3	XP_012040331.1	3 - 3	2878	111
	XM_012184942.2	XP_012040332.1	3 - 3	2882	111
	XM_012184943.3	XP_012040333.1	3 - 3	3006	111
	XM_012184944.3	XP_012040334.1	2 - 2	4791	111
	XM_012184946.2	XP_012040336.1	2 - 2	2706	111
	XM_012184947.3	XP_012040337.1	2 - 2	2831	111
GenBank mRNA	KT180155.1	-	1 - 1	2621	111