The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001042738.1 | NP_001036203.1 | - | 536 | 145 |
| XM_015120065.2 | XP_014975551.2 | - | 690 | 145 | |
| XM_015120066.2 | XP_014975552.1 | - | 574 | 145 | |
| GenBank mRNA | L16556.1 | - | - | 536 | 145 |