The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_031041.1 | NP_112303.1 | 3 - 3 | 142 | 76 |
| GenBank mRNA | BC085345.1 | - | 3 - 3 | 147 | 76 |
| FQ229769.1 | - | 3 - 3 | 130 | 76 | |
| FQ231337.1 | - | 3 - 3 | 172 | 76 | |
| X65948.1 | - | 3 - 3 | 142 | 76 | |