The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001127652.1 | NP_001121124.1 | - | 196 | 71 |
| GenBank mRNA | BC158762.1 | - | - | 196 | 71 |
| FQ216242.1 | - | 2 - 2 | 234 | 71 | |
| FQ216641.1 | - | 2 - 2 | 236 | 71 | |
| FQ224910.1 | - | 2 - 2 | 234 | 71 | |
| X59736.1 | - | 2 - 2 | 224 | 71 | |