The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_032058.2 | NP_114447.2 | 5 - 5 | 705 | 274 |
| GenBank mRNA | BC070506.1 | - | 5 - 5 | 705 | 274 |
| FQ232990.1 | - | 5 - 5 | 714 | 274 | |
| U31880.1 | - | 5 - 5 | 708 | 274 | |
| U83914.1 | - | 5 - 5 | 676 | 274 | |