The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_003360208.4 | XP_003360256.3 | - | 362 | 85 |
| GenBank mRNA | AK343710.1 | - | 3 - 3 | 341 | 85 |
| AK348381.1 | - | 3 - 3 | 342 | 85 | |
| AK389729.1 | - | 3 - 3 | 350 | 85 | |
| AY439280.1 | - | 2 - 2 | 53 | 85 | |
| AY609957.1 | - | 3 - 3 | 339 | 85 | |