The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001256774.1 | NP_001243703.1 | 2 - 2 | 301 | 73 |
| GenBank mRNA | AK394989.1 | - | 2 - 2 | 332 | 73 |
| AY934805.2 | - | 2 - 2 | 212 | 73 | |
| EF179159.1 | - | 2 - 2 | 153 | 73 | |
| FJ156753.1 | - | 2 - 2 | 301 | 73 | |