The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001007517.1 | NP_001007518.1 | 4 - 4 | 349 | 103 |
| GenBank mRNA | AK343626.1 | - | 4 - 4 | 365 | 103 |
| AK344883.1 | - | 4 - 4 | 351 | 103 | |
| AK392358.1 | - | 4 - 4 | 354 | 103 | |
| AK400056.1 | - | 4 - 4 | 423 | 103 | |
| AY786556.1 | - | 4 - 4 | 349 | 103 | |