The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001031795.1 | NP_001026965.1 | 4 - 4 | 449 | 121 |
| XM_013988634.2 | XP_013844088.1 | - | 598 | 121 | |
| GenBank mRNA | AK235782.1 | - | 4 - 4 | 585 | 121 |
| AK348421.1 | - | 4 - 4 | 537 | 121 | |
| AY610421.1 | - | 4 - 4 | 525 | 121 | |
| AY996815.1 | - | 4 - 4 | 449 | 121 | |