The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001048069.1 | NP_001041534.1 | 3 - 3 | 246 | 101 |
| XM_013980752.2 | XP_013836206.1 | - | 365 | 116 | |
| GenBank mRNA | AK233714.1 | - | 3 - 3 | 331 | 101 |
| AY610380.1 | - | 3 - 3 | 286 | 101 | |