The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_214087.1 | NP_999252.1 | 5 - 5 | 1047 | 99 |
| XM_021068533.1 | XP_020924192.1 | - | 1166 | 99 | |
| XM_021068534.1 | XP_020924193.1 | - | 1164 | 96 | |
| XM_021068535.1 | XP_020924194.1 | - | 1165 | 96 | |
| GenBank mRNA | AB049760.1 | - | 5 - 5 | 1047 | 99 |
| AF203443.1 | - | 5 - 5 | 1032 | 99 | |
| AF455811.1 | - | 4 - 4 | 725 | 96 | |