The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_214237.2 | NP_999402.1 | 2 - 2 | 255 | 92 |
| XM_021068544.1 | XP_020924203.1 | - | 341 | 92 | |
| GenBank mRNA | AK344003.1 | - | 2 - 2 | 225 | 92 |
| EW238422.2 | - | 2 - 2 | 247 | 92 | |
| K01940.1 | - | 2 - 2 | 250 | 92 | |