The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_214376.1 | NP_999541.1 | 5 - 5 | 847 | 92 |
GenBank mRNA | AY028310.1 | - | 5 - 5 | 847 | 92 |