The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_005618914.2 | XP_005618971.1 | 9 - 9 | 946 | 143 |
| XM_005618915.3 | XP_005618972.1 | 9 - 9 | 947 | 143 | |
| XM_005618917.3 | XP_005618974.1 | 9 - 9 | 947 | 143 | |
| XM_005618918.3 | XP_005618975.1 | 9 - 9 | 946 | 143 | |
| XM_005618919.3 | XP_005618976.1 | 9 - 9 | 946 | 143 | |
| XM_014112826.2 | XP_013968301.1 | 9 - 9 | 1124 | 143 | |
| XM_022417459.1 | XP_022273167.1 | 9 - 9 | 851 | 143 | |
| XM_022417460.1 | XP_022273168.1 | 9 - 9 | 1045 | 143 | |
| XM_536360.5 | XP_536360.3 | 9 - 9 | 946 | 143 | |