The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001083367.1 | NP_001076836.1 | 4 - 4 | 333 | 158 |
| XM_005631051.3 | XP_005631108.1 | 5 - 5 | 513 | 158 | |
| XM_005631052.3 | XP_005631109.1 | 5 - 5 | 513 | 158 | |
| XM_022405071.1 | XP_022260779.1 | 5 - 5 | 391 | 158 | |
| GenBank mRNA | EF427641.1 | - | 4 - 4 | 333 | 158 |