The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001287105.1 | NP_001274034.1 | 2 - 2 | 230 | 141 |
| NM_001287106.1 | NP_001274035.1 | 2 - 2 | 201 | 141 | |
| GenBank mRNA | CO658560.1 | - | 4 - 4 | 380 | 141 |
| DN863506.1 | - | 4 - 4 | 395 | 141 | |
| DN871196.1 | - | 2 - 2 | 201 | 141 | |
| GR889127.1 | - | 2 - 2 | 231 | 141 | |
| GR894797.1 | - | 2 - 2 | 179 | 141 | |