Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: br
Entrez Gene ID: 44505
Gene Name: broad
Gene Aliases: 2B5, 2Bc, A18, BR, BR-C, BR-C Z1, BR-Z1, BR-Z2, BR-Z3, BR-Z4, BR-c, BRC, BRC-Z1, BRC-Z2, BRC-Z3, BRC-Z4, BR_C, Br, Br-C, Br-C Z2, Br-C-Z3, Br-Z2, Br-Z4, Br-c, BrC, BrC-Z1, BrZ3, CG11491, CG11509, CG11511, CG11514, Dmel\CG11491, Dmel_CG11491, EG:123F11.1, EG:17A9.1, EG:25D2.1, PP1, PP2, Z1, Z2, Z3, Z4, br-C, br-Z1, br-Z2, br-Z3, br-Z4, br-c, de12, ecs, l(1)2Ba, l(1)2Bab, l(1)2Bad, l(1)2Bb, l(1)2Bc, l(1)2Bd, l(1)ESHS5, l(1)G0018, l(1)G0042, l(1)G0284, l(1)G0284a, l(1)G0318, l(1)G0401, l(1)PP1, l(1)d norm-12, l(1)d.norm.1, l(1)dn1, l(1)n34, l(1)npr-1, l(1)npr1, l(1)pp-1, l(1)pp-2, l(1)pp1, l(1)pp2, l(1)ts132, l(1)ts144, l(1)ts358, l(1)ts376, npr, npr-1, npr1, nprl, nrp, nrp1, o.c.c., rbp, rdp, rds, uq
Chromosome Location: Chr.X: 1575132 - 1664869 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0283451

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001144672.3	NP_001138144.1	3 - 4	1370	78
	NM_001169167.2	NP_001162638.2	3 - 4	1253	78
	NM_001201596.3	NP_001188525.1	3 - 4	1220	78
	NM_001201597.1	NP_001188526.1	3 - 4	1014	78
	NM_001201598.1	NP_001188527.1	3 - 4	1347	78
	NM_001258533.1	NP_001245462.1	4 - 5	1352	78
	NM_001272205.1	NP_001259134.1	4 - 5	1300	78
	NM_001382013.1	NP_001368968.1	3 - 4	1220	78
	NM_080020.4	NP_524759.2	3 - 4	1220	78
	NM_166893.3	NP_726749.2	3 - 4	1220	78
	NM_166894.4	NP_726750.1	3 - 4	1220	78
	NM_166895.2	NP_726751.2	3 - 4	1046	78
	NM_166896.3	NP_726752.1	3 - 4	1220	78
	NM_166897.2	NP_726753.1	3 - 4	932	78
	NM_166898.2	NP_726754.1	3 - 4	1220	78
GenBank mRNA	AY069756.1	-	3 - 4	1220	78
	BT125021.1	-	4 - 5	1352	78
	X54665.1	-	-	759	78