The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_008218.2 | NP_032244.2 | 2 - 2 | 252 | 131 |
GenBank mRNA | AK003077.1 | - | 2 - 2 | 250 | 131 |
AK010923.1 | - | 2 - 2 | 250 | 131 | |
AK010927.1 | - | 2 - 2 | 251 | 131 | |
AK010933.1 | - | 2 - 2 | 250 | 131 | |
AK010937.1 | - | 2 - 2 | 253 | 131 | |
AK010987.1 | - | 2 - 2 | 250 | 131 | |
AK011018.1 | - | 2 - 2 | 252 | 131 | |
AK011023.1 | - | 2 - 2 | 249 | 131 | |
AK011025.1 | - | 2 - 2 | 252 | 131 | |
AK011028.1 | - | 2 - 2 | 249 | 131 | |
AK011055.1 | - | 2 - 2 | 253 | 131 | |
AK011056.1 | - | - | 788 | 131 | |
AK011063.1 | - | 2 - 2 | 257 | 131 | |
AK011076.1 | - | 2 - 2 | 250 | 131 | |
AK011116.1 | - | - | 763 | 131 | |
AK012329.1 | - | 2 - 2 | 250 | 131 | |
AK012755.1 | - | 2 - 2 | 250 | 131 | |
AK171893.1 | - | 2 - 2 | 250 | 131 | |
BC043020.1 | - | 2 - 2 | 232 | 131 | |
L75940.1 | - | 2 - 2 | 131 |
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001083955.1 | NP_001077424.1 | - | 249 | 131 |
GenBank mRNA | AK002222.1 | - | - | 252 | 131 |
AK005434.1 | - | - | 250 | 131 | |
AK010422.1 | - | - | 250 | 131 | |
AK010938.1 | - | - | 251 | 131 | |
AK011030.1 | - | - | 250 | 131 | |
AK011073.1 | - | - | 250 | 131 | |
AK011121.1 | - | - | 250 | 131 | |
AK012348.1 | - | - | 250 | 131 | |
AK019138.1 | - | - | 251 | 131 | |
AK028173.1 | - | - | 250 | 131 | |
AK076068.1 | - | - | 250 | 131 | |
CF740193.1 | - | - | 236 | 131 | |
M26895.1 | - | - | 223 | 131 |