The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001134343.1 | NP_001127815.1 | 11 - 11 | 1147 | 62 |
| GenBank mRNA | AF307856.1 | - | 2 - 2 | 149 | 62 |
| AK239476.1 | - | 5 - 5 | 527 | 62 | |
| AK239820.1 | - | 11 - 11 | 1475 | 62 | |
| AK393036.1 | - | 11 - 11 | 1265 | 62 | |
| AK395278.1 | - | 11 - 11 | 1271 | 62 | |
| AK398865.1 | - | 11 - 11 | 1321 | 62 | |
| AY610096.1 | - | 11 - 11 | 1335 | 62 | |
| EU868832.1 | - | 11 - 11 | 1147 | 62 | |