The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001044530.1 | NP_001037995.1 | 12 - 12 | 1352 | 100 |
| XM_013980932.2 | XP_013836386.1 | - | 1292 | 100 | |
| GenBank mRNA | AK235833.1 | - | 12 - 12 | 1347 | 100 |
| AK390423.1 | - | 12 - 12 | 1361 | 100 | |
| AM051354.1 | - | 12 - 12 | 1352 | 100 | |