The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_213960.1 | NP_999125.1 | 7 - 7 | 746 | 75 |
| XM_005652948.3 | XP_005653005.1 | - | 875 | 75 | |
| XM_005652949.3 | XP_005653006.1 | - | 853 | 75 | |
| XM_005652950.3 | XP_005653007.1 | - | 913 | 75 | |
| XM_021080765.1 | XP_020936424.1 | - | 894 | 75 | |
| GenBank mRNA | AF268461.1 | - | 7 - 7 | 746 | 75 |
| AK235603.1 | - | 8 - 8 | 817 | 75 | |
| AK391290.1 | - | 8 - 8 | 828 | 75 | |
| AK392730.1 | - | 8 - 8 | 789 | 75 | |
| AK393361.1 | - | 8 - 8 | 817 | 75 | |
| AK393578.1 | - | 8 - 8 | 801 | 75 | |
| AK395118.1 | - | 8 - 8 | 821 | 75 | |
| AK400488.1 | - | 8 - 8 | 886 | 75 | |