The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_005618572.3 | XP_005618629.1 | 2 - 2 | 779 | 108 |
| XM_005618574.2 | XP_005618631.1 | 3 - 3 | 570 | 108 | |
| XM_005618575.2 | XP_005618632.1 | 2 - 2 | 385 | 108 | |
| XM_022417162.1 | XP_022272870.1 | 3 - 3 | 1953 | 108 | |
| XM_022417163.1 | XP_022272871.1 | 4 - 4 | 2016 | 108 | |
| XM_022417164.1 | XP_022272872.1 | 4 - 4 | 727 | 108 | |
| XM_845799.4 | XP_850892.1 | 2 - 2 | 507 | 108 | |