Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Spt-I
Entrez Gene ID: 36448
Gene Name: Serine palmitoyltransferase subunit I
Gene Aliases: CG4016, Dmel\CG4016, Dmel_CG4016, SPT, SPT-I, SPT1, Spt1, dSpt1, l(2)49Fb, l(2)SH1626, l(2)SH2 1626, lcb1
Chromosome Location: Chr.2R: 13168498 - 13170744 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0086532

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_136998.2	NP_610842.1	4 - 5	1304	111
	NM_165967.4	NP_725255.1	5 - 6	1376	111
	NM_165968.4	NP_725256.1	5 - 6	1346	111
GenBank mRNA	BT010234.1	-	5 - 6	1344	111