The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_005131.2 | NP_005122.2 | 21 - 21 | 2066 | 140 |
| XM_011525772.2 | XP_011524074.1 | 21 - 21 | 2087 | 140 | |
| XM_011525773.1 | XP_011524075.1 | 22 - 22 | 2117 | 140 | |
| XM_011525774.2 | XP_011524076.1 | 11 - 11 | 2658 | 140 | |
| XM_017026103.1 | XP_016881592.1 | 18 - 18 | 3607 | 140 | |
| GenBank mRNA | AK055354.1 | - | 10 - 10 | 2737 | 140 |
| AK123530.1 | - | 8 - 8 | 2203 | 140 | |
| AK225067.1 | - | 21 - 21 | 2041 | 139 | |
| AK225709.1 | - | 21 - 21 | 2030 | 140 | |
| BC010381.1 | - | 21 - 21 | 2056 | 139 | |
| L36529.1 | - | 21 - 21 | 2040 | 140 | |