The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000167.5 | NP_000158.1 | 19 - 19 | 4244 | 85 |
| NM_001128127.2 | NP_001121599.1 | 20 - 20 | 4331 | 85 | |
| NM_001205019.1 | NP_001191948.1 | 21 - 21 | 4349 | 85 | |
| NM_203391.3 | NP_976325.1 | 20 - 20 | 4262 | 85 | |
| GenBank mRNA | AW300142.1 | - | 1 - 1 | 85 | |