The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_011629.3 | NP_035759.3 | 10 - 10 | 1385 | 236 |
| GenBank mRNA | AK146891.1 | - | 10 - 10 | 1398 | 236 |
| AK149374.1 | - | 10 - 10 | 1300 | 236 | |
| BC090662.1 | - | 10 - 10 | 1361 | 236 | |
| BC094580.1 | - | 10 - 10 | 1303 | 236 | |
| L26957.1 | - | 9 - 9 | 1194 | 236 | |
| U28265.1 | - | 9 - 9 | 1194 | 236 | |
| U30482.1 | - | 9 - 9 | 1194 | 236 | |
| Y11436.1 | - | 9 - 9 | 1833 | 236 | |