Close X

SiRNA Validation Data

siRNA ID:

111348

Gene ID:

8702

Transcript(s):

NM_003778.3 NM_212543.1 XM_005247855.1 XM_006713798.2 XM_006713799.2 XM_006713800.1 XM_006713801.2 XM_011513260.1 XM_017007401.1

Validation Results:

28%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00186850_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.