SiRNA Validation Data

siRNA ID:

143975

Gene ID:

Transcript(s):

NM_005026.3 XM_006710687.2 XM_006710689.2 XM_017001476.1 XM_017001477.1 XM_017001478.1 XM_017001479.1 XM_017001480.1 XM_017001481.1 XM_017001482.1 XM_017001483.1 XM_017001484.1 XM_017001485.1

Validation Results:

9%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00192399_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.