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SiRNA Validation Data

siRNA ID:

s10021

Gene ID:

5058

Transcript(s):

NM_001128620.1 NM_002576.4 XM_011545080.2 XM_011545081.2 XM_011545082.2 XM_011545083.2 XM_011545084.2 XM_017017846.1 XM_017017847.1 XM_017017848.1 XM_017017849.1 XM_017017850.1

Validation Results:

11%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00176815_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.