Close X

SiRNA Validation Data

siRNA ID:

s11492

Gene ID:

5754

Transcript(s):

NM_001270398.1 NM_002821.4 NM_152880.3 NM_152881.3 NM_152882.3 NR_072997.1 NR_072998.1 XM_011514765.2 XM_011514766.2

Validation Results:

6%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177173_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.