SiRNA Validation Data

siRNA ID:

s3911

Gene ID:

1606

Transcript(s):

NM_001345.4 NM_201444.2 NM_201445.1 NM_201554.1 XM_005268688.2 XM_005268689.2 XM_005268690.2 XM_011537991.2 XM_011537993.2 XM_011537995.2 XM_017018900.1 XM_017018901.1 XM_017018902.1 XM_017018903.1 XM_017018904.1 XM_017018905.1 XM_017018906.1 XM_017018907.1 XM_017018908.1 XM_017018909.1

Validation Results:

5%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00176278_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.