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SiRNA Validation Data

siRNA ID:

s9669

Gene ID:

4864

Transcript(s):

NM_000271.4 XM_005258277.1 XM_005258278.4 XM_005258279.2 XM_006722479.3 XM_011526015.1 XM_017025784.1 XM_017025785.1 XM_017025786.1 XM_017025787.1

Validation Results:

18%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00264835_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.