SiRNA Validation Data



Gene ID:

11163 440672 170688


NM_001301022.1 NM_001301023.1 NM_001301024.1 NM_019094.5 NM_199040.3 XM_011537794.2 XM_011537795.2 XM_011537796.2 NR_002212.4 NR_104005.1

Validation Results:

29%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00819603_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.