SiRNA Validation Data



Gene ID:



NM_001272042.1 NM_001272043.1 NM_001272044.1 NM_001272060.1 NM_003161.3 XM_011525101.2 XM_011525102.2 XM_011525103.2 XM_017024929.1 XM_017024930.1 XM_017024931.1 XM_017024932.1 XM_017024933.1

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00177357_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.