SiRNA Validation Data

siRNA ID:

s159

Gene ID:

1635

Transcript(s):

NM_001012732.1 NM_001921.2 XM_005262778.3 XM_005262779.3 XM_005262780.3 XM_005262781.4 XM_005262782.3 XM_006714115.3 XM_006714116.3 XM_011531674.2 XM_011531675.2 XM_011531676.2 XM_017007816.1 XM_017007817.1 XM_017007818.1 XM_017007819.1 XM_017007820.1 XM_017007821.1 XM_017007822.1 XM_017007823.1 XM_017007824.1

Validation Results:

11%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00157241_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.