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SiRNA Validation Data

siRNA ID:

607

Gene ID:

3717

Transcript(s):

NM_001322194.1 NM_001322195.1 NM_001322196.1 NM_001322198.1 NM_001322199.1 NM_004972.3

Validation Results:

26%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.