SiRNA Validation Data



Gene ID:



NM_001001935.2 NM_001001937.1 NM_001257334.1 NM_001257335.1 NM_004046.5 XM_017025789.1

Validation Results:

13%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00818620_g1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.