SiRNA Validation Data



Gene ID:



NM_000964.3 NM_001024809.3 NM_001145301.2 NM_001145302.2 XM_005257552.4 XM_005257553.1 XM_005257554.1 XM_011525095.1 XM_011525096.1 XM_017024920.1

Validation Results:

19%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00230907_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.