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SiRNA Validation Data

siRNA ID:

s10252

Gene ID:

5163

Transcript(s):

NM_001278549.1 NM_002610.4 NR_103729.1 NR_103731.1 XM_006712594.2 XM_011511343.2 XM_011511344.2 XM_011511345.2 XM_011511347.2

Validation Results:

20%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00176853_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.