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SiRNA Validation Data

siRNA ID:

s13570

Gene ID:

6789

Transcript(s):

NM_006282.3 XM_005260530.2 XM_005260531.3 XM_005260532.3 XM_005260533.2 XM_011529018.2 XM_017028029.1 XM_017028030.1 XM_017028031.1 XM_017028032.1

Validation Results:

16%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00178979_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.