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SiRNA Validation Data

siRNA ID:

s3633

Gene ID:

1456

Transcript(s):

NM_001031812.3 NM_001044723.2 NM_001270572.1 NM_004384.4 XM_005271891.1 XM_005271892.2 XM_005271894.2 XM_005271895.2 XM_005271896.1 XM_017009069.1 XM_017009070.1

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177858_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.