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SiRNA Validation Data

siRNA ID:

s545

Gene ID:

4363

Transcript(s):

NM_004996.3 XM_011522497.1 XM_011522498.2 XM_017023237.1 XM_017023238.1 XM_017023239.1 XM_017023240.1 XM_017023241.1 XM_017023242.1 XM_017023243.1

Validation Results:

18%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00219905_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.