SiRNA Validation Data

siRNA ID:

s12651

Gene ID:

6389 255812 727956

Transcript(s):

NM_001294332.1 NM_004168.3 XM_005248331.3 XM_011514072.1 XM_011514073.1 XM_017009685.1 NR_003264.2 NR_003265.3

Validation Results:

6%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00188166_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.