SiRNA Validation Data

siRNA ID:

1184

Gene ID:

Transcript(s):

NM_001042482.1 NM_022445.3 XM_005249970.1 XM_011516031.1 XM_011516032.2 XM_011516033.2 XM_011516034.2 XM_011516035.2 XM_011516037.2 XM_011516039.2 XM_011516040.2 XM_011516041.2 XM_011516043.1 XM_011516044.1 XM_011516046.1 XM_017011969.1 XM_017011970.1 XM_017011971.1 XM_017011972.1 XM_017011973.1 XM_017011974.1 XM_017011975.1 XM_017011976.1 XM_017011977.1 XM_017011978.1 XM_017011979.1 XM_017011982.1 XM_017011985.1 XM_017011986.1

Validation Results:

12%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00223714_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.