Close X

SiRNA Validation Data

siRNA ID:

143005

Gene ID:

5569

Transcript(s):

NM_006823.3 NM_181839.2 XM_017013620.1 XM_017013621.1 XM_017013622.1

Validation Results:

5%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00738983_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.