SiRNA Validation Data

siRNA ID:

44790

Gene ID:

Transcript(s):

NM_001204502.1 NM_001204503.1 NM_001278638.1 NM_001459.3 XM_005258680.4 XM_005258681.4 XM_005258682.4 XM_005258683.4 XM_006723116.3 XM_011526675.2 XM_011526676.2 XM_011526677.2 XM_011526678.2 XM_011526680.2 XM_011526682.2 XM_017026532.1

Validation Results:

8%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00181740_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.